Development of Eighteen Polymorphic Microsatellite Markers in Schizopygopsis Younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China

Eighteen polymorphic microsatellite primers were developed for an endemic Tibetan fish Schizopygopsis younghusbandi, using an enrichment genomic library. The ability of the primers to generate reliable polymorphic PCR products was tested with the genomic DNA of 29 individuals of S. younghusbandi from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus in the polymorphic loci ranged from 3 to 30, with expected heterozygosity ranging from 0.527 to 0.937. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.