Long-Term Storage Of Fish Tissue Samples For Genetic Analysis

Successful long-term storage of fish tissue samples is required for future genetic analysis. Usually fish tissues are preserved in one of the 3 ways:  -80^oC, 95% ethanol or Dimethyl sulfoxide (DMSO)/NaCl solution. Although these traditional methods preserve tissues that can yield high quality and quantity DNA extractions, they are expensive to operate, space-consuming or do potential harm to human being. In this study, we tried to find an alternate method to store fish tissue samples at room temperature. The ideal new method will yield the same or higher quality and quantities of extractable DNA in the simplest, safest, and most convenient method.  We investigated a total of 7 storage methods – 3 with the freeze-drying treatment and 4 without it. By comparison the tissue samples stored in the treatment of freeze-dry with envelope gave the second highest DNA yield with the lowest genotype failure rate. As a result, freeze-dry with envelope method is considered as an alternative ideal method.