Airsbase: A Blastable Database of DNA Sequences for Species Detection, and Population, Phylogeographic and Phylogenetic Caracterization of Aquatic Invasive and Rare Species

Monday, August 18, 2014: 2:10 PM
304B (Centre des congrès de Québec // Québec City Convention Centre)
Tim L. King , Leetown Science Center, U.S. Geological Survey, Kearneysville, WV
John Miller , School of Forest Resources,, Penn State University, University Park, PA
Aaron Aunins , Leetown Science Center, Cherokee Nation Technology Solutions, Kearneysville, WV
Jay Stauffer Jr. , School of Forest Resources, Pennsylvania State University, University Park, PA
James Grazio , Department of Environmental Protection, Erie, PA
Recent development of ultra-high throughput sequencing technologies can produce epic numbers of DNA sequence reads at an affordable cost, and has boosted the number and scope of DNA sequencing projects.  Actual application of such tools has lagged significantly behind technical advances, and relatively few examples exist of genome-scale methods that have directly influenced management decisions.  We present a status/update of our efforts to create a database containing thousands of annotated contigs and singleton sequences for many aquatic invasive and rare species.  We will discuss the nuclear and mitogenomic results for two species of Asian carp, European rudd, Northern snakehead, round and tubenose gobies, and four North American sturgeon species.  AIRSbase will allow researchers to BLAST the results of environmental samples to efficiently detect the presence of invasive and/or rare species and to characterize ecosystem structure and function.  Moreover, it will provide thousands of annotated gene sequences and marker loci for assessing kinship, population delineation, and phylogeographic and phylogenetic resolution. The assembled and annotated genome sequences,  gene ontologies, the complete mitochondrial genome sequence, single nucleotide polymorphisms (SNPs), and microsatellite markers for each rare and invasive species incorporated into the database will be made available in the form of BLASTable database.