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P-117
"Searching for Potential Funcitonality of Divergent SNP Markers from Lake Erie Walleye Spawning Stocks"

Monday, August 18, 2014

Exhibit Hall 400AB (Centre des congrès de Québec // Québec City Convention Centre)

Benjamin A. Bolam , Department of Evolution, Ecology & Organismal Biology - Aquatic Ecology Laboratory, The Ohio State University, Columbus, OH

Kuan-Yu Chen , Aquatic Ecology Laboratory, Dept. of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, OH

Elizabeth A. Marschall , Evolution, Ecology, and Organismal Biology, Aquatic Ecology Laboratory, The Ohio State University, Columbus, OH

Michael G. Sovic , Dept. of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, OH

H. Lisle Gibbs , Dept. of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, OH

Stuart A. Ludsin , Evolution, Ecology, and Organismal Biology, Aquatic Ecology Laboratory, The Ohio State University, Columbus, OH

Next-generation sequencing techniques offer a means to identify large numbers of divergent

single nucleotide polymorphisms (SNPs) within fish population sub-groups (i.e., stocks). In turn,

linking these divergent SNP loci to functionality could help delineate the selective forces that

drive population (and stock) structure. Towards this end, we used restriction-site associated

DNA sequencing techniques to identify 345 SNP loci that occurred in all sampled individuals

from three Lake Erie walleye (Sander vitreus) spawning stocks (Sandusky River, Maumee River,

and Ohio reef complex). We then used F_ST-outlier analyses to identify 23 SNP

loci that significantly differed among the stocks. To begin to assess whether these loci were

associated with functionality upon which selection might occur, we compared their sequences

to those in GenBank and Ensembl. Using the NCBI’s Basic Local Alignment Search Tool (BLAST),

we associated 4 of the 23 loci with protein coding genes: ankrd16 (protein folding gene); optc

(ocular structural gene); unc5c (neural structural gene; and nbeal2-like(blood thrombocyte

gene). Because these coding DNA sequences are involved in various morphological and

immunological development processes in other fishes, they may serve as candidate loci to help

explain selective forces driving differences among Lake Erie walleye spawning stocks.