P-193
Brood Source Identification and the Effects of Supplementation on Muskellunge in the Great Lakes

Patrick Hanchin , Charlevoix Fisheries Station, Michigan Department of Natural Resources, Charlevoix, MI
Keith Turnquist , Molecular Conservation Genetics Laboratory, Wisconsin Cooperative Fishery Research Unit, University of Wisconsin-Stevens Point, Stevens Point, WI
Brian L. Sloss , College of Natural Resources, University of Wisconsin - Stevens Point, Stevens Point, WI
Kevin Kapuscinski , Fisheries and Wildlife, Lake Superior State University
John M. Farrell , Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, Syracuse, NY
Loren M. Miller , Minnesota Department of Natural Resources, Saint Paul, MN
Kim Scribner , Department of Fisheries & Wildlife and Department of Zoology, Michigan State University, East Lansing, MI
Chris Wilson , Aquatic Research and Monitoring Section, Ontario Ministry of Natural Resources, Peterborough, ON, Canada
Current Muskellunge (Esox masquinongy) management in Great Lakes States and Provinces call for stocking native Great Lakes Muskellunge to re-establish sustainable populations that have previously experienced population declines or extirpation.  Accordingly, native Great Lakes Muskellunge brood sources must be identified or established to meet that need.  Managers seeking to develop future Muskellunge brood stocks benefit from knowledge of stock structure to better match the origin of hatchery fish to stocking locations.  Therefore our objectives were to (1) determine if the genetic structure of non-admixed Great Lakes Muskellunge populations is consistent with a genetic stock model that can be described in terms of genetic stock identification and degree of stock isolation for the identification of potential brood sources, and (2) determine if significant admixture is present in Great Lakes Muskellunge populations consistent with introgression between stocked and resident Great Lakes Muskellunge.  Fourteen microsatellite loci were used to characterize genetic diversity and structure of >1,800 Muskellunge from >40 locations throughout the Great Lakes and associated inland drainages.  Genetic diversity and molecular variance will be compared within and among various genetic structure models to identify possible influences of historical stocking and, ultimately, potential brood sources for Great Lakes Muskellunge supplementation efforts.