P-39 Using Plasma Vitellogenin Levels to Predict Reproductive Success of Reconditioned Steelhead Kelts in an Artificial Spawning Channel

Jeff Stephenson , Columbia River Inter-Tribal Fish Commission, Hagerman, ID
Douglas Hatch , Fish Science, Columbia River Inter-Tribal Fish Commission, Portland, OR
David Fast , Yakama Nation, Toppenish, WA
William Bosch , Fisheries, Yakama Nation, Toppenish, WA
Joe Blodgett , Yakama Nation YKFP, Toppenish, WA
Jeff Trammell , Yakama Nation, Toppenish, WA
Andrew Pierce , Fish Science, Columbia River Inter-Tribal Fish Commission, Portland, OR
We examined the reproductive success of reconditioned kelts placed in an artificial spawning channel. Post-spawn kelts were captured in the spring at the Chandler Juvenile Collection Facility on the Yakima River and reconditioned for 3-4 months. Prior to release, fish were categorized as either maturing or non maturing based on blood vitellogenin levels. Fish were then released into the artificial spawning channel to over-winter until spawning the following spring. Parents and progeny were sampled for genetic analysis using a suite of microsatellite markers. Parentage assignment allowed a comparison of relative reproductive success of fish assigned as either maturing or non maturing based on the blood vitellogenin levels.