Freshwater ecosystems are increasingly challenged by threats including climate and land use changes.  Planning for and implementing strategies to cope with changes to freshwater ecosystems can be difficult for resource managers.  These changes are likely to affect aquatic systems by altering flow, temperature, and sediment regimes as well as concentrations of nutrients and toxins.  Habitats (reach/network scale) are not equally vulnerable to changing environmental conditions, and fish perceive these effects differently.  Effects of climate change on water temperature and flow regimes are expected to alter freshwater habitats in lakes and streams and lead to changes in fish abundance and distribution, with declines predicted for many temperature specialists, often native species.  Conversion of catchment land area to urban and agricultural uses has been directly linked with disruptions in stream integrity and loss of fish diversity across the United States.  In combination, changing climate and land use patterns pose particularly serious threats to aquatic systems.  This symposium will examine the potential effects of climate and land use change across multiple spatial scales in the conterminous United States. We invite contributions that: 1) demonstrate the transformation of aquatic habitats due to historical changes of climate and land use, or 2) incorporate downscaled Atmosphere-Ocean General Circulation Models and projected land use models to identify how future climate and land use will affect fish distributions, fish behavior (spawning, foraging, interactions, etc.), and fish habitat in lakes and streams at various spatial scales.  Specifically, we will report on research based on approaches that can serve as a framework for future investigations to identify the large scale effects of changing patterns of climate and land use on fish and their habitats.  The results from this symposium will identify important habitats for conservation or restoration and especially potential management options for managing these changes in our aquatic landscape.