This symposium is a continuation of the Mississippi-Yangtze River Basins Symposium (MYRIBS) series, which started in Little Rock, Arkansas, USA during the 2013 American Fisheries Society annual meeting, and continued in Wuhan, Hubei, China during the 2015 China Society of Fisheries Fishery Resources and Environment annual meeting. The current symposium will continue fostering and promoting the Mississippi-Yangtze communications, but also will encourage exchanges between scientists working in other large river basins in both United States and China, and large rivers in other countries or continents. Many methods and techniques, including biotelemetry, stoichiometry, numerous fish sampling gears and designs (e.g., electrofishing, the United Fishing Method), and geospatial analyses among others have been applied to managing and conserving the fishery resources and environments of large river basins throughout the world. However, applications of these methods have rarely been compared and evaluated across different river basins. Moreover, some of the methods and techniques used in some river basins have not been sufficiently assessed with regard to their application in other river basins. This symposium aims to fill these information gaps by addressing some of the common issues related to the methods and techniques used to assess fisheries resources and the environment in large river basins. It will also emphasize exchanges and transfers of information on different methods and techniques among large river basins. Paired and/or co-authored presentations among different river basins and discussions will focus on these topics to manage and conserve large river basin fishery resources and environment. Each presentation will cover at least one of these methods or techniques applied in the large river basin. A final group synthesis will discuss methods exchanges and transfers among basins. Symposium attendees will be provided with the opportunity to learn these methods and techniques, and their transfers across different basins.